Department of Human Genetics

UNIVERSITY of DEBRECEN

Research fields

Microbiomics research group

Members: Prof. Dr. Sándor Biró, Dr. Melinda Paholcsek, Gábor Fidler, Emese Tolnai, Anna Anita Rácz, Péter Zsombor Fauszt, Péter Dávid, Klára Plébán Komárominé, Sándor Újszászi
We perform 16S and 18S rRNA gene amplification-based next-generation sequencing (NGS) to investigate the composition of different microbiota (human and animal feces specimens, soil, water and other environmental samples). Running Projects: i) Student Smart Stool Project, ii) Sport Stool Project. iii) We also investigate the contribution of the microbiome for estimating Tokaj Wine’s fermentation.
homepage: http://smartstool.eu/index.php/hu/
 

Study the role of miRNAs in the carcionogenic action of estrogen molecules

Participants: Melinda Szilágyi-Bónizs, Bálint Nagy, Éva Márton, Katalin Trefán

MicroRNAs (miRNAs) are small non-coding RNA molecules, which play important role in posttranscriptional gene regulation. They are involved in the regulation of several biological processes including development, differentiation, apoptosis or proliferation. MicroRNAs are also present in body fluids due to their active transport from the cells that suggest their role in cell-cell communication. MiRNAs has become the focus of interest in cancer studies recently due to the fact that these molecules proved to have various roles in oncogenesis as oncomirs or tumor suppressors. The carcinogenic action of estrogen molecules is a well-known phenomenon in breast cancer, however, limited information is available about their contribution to the development of ovarian cancer. In our studies we would like to shed light on the role of miRNAs in the carcinogenic effect of estrogen molecules in human epithelial ovarian cell cultures.

Monoclonal antibody proteomics

Members: Laszlo Takacs, Ilona Tornyi, Biosystems International Ltd.: József Lázár, Lora Szilágyi, Anna Gáll-Debreczeni, Zsombor Lestár

One form of proteomics research is affinity proteomics, monoclonal antibody proteomics is one form of affinity proteomics.
We focus on applied translational research; our goals are the development of blood tests that aid cancer diagnosis and the understanding of the underlying molecular mechanism. To achieve the goal our laboratory, in collaboration with Biosystems International Ltd., established the epitome profiling technology.  We prepared monoclonal antibody libraries directed against natural epitopes of the human blood plasma, we then applied the libraries onto biochips (Randox Ltd. Northern Ireland). We profile close to 300 epitopes of about 100 proteins. With the aid of machine learning algorithms we develop multivariate index assays for the early detection of breast, colon, ovarian and lung cancers. The panels run on the Evidence Investigator platform and on the classic 96-well ELISA plates. The latter lung cancer test (QPLC96w) is sold by BSI, the university laboratory is involved in research addressed towards the understanding of the molecular basis of the phenomenon.  
We published 11 peer reviewed scientific papers on the topic (papers), and BSI filed >10 patents (patents).